Plasmid

Part:BBa_K4344040:Design

Designed by: Marcel Pott   Group: iGEM22_Heidelberg   (2022-09-29)


pEGFP-C1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1359
    Illegal XbaI site found at 1402
    Illegal PstI site found at 1364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1359
    Illegal NheI site found at 591
    Illegal PstI site found at 1364
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1359
    Illegal BglII site found at 1339
    Illegal BamHI site found at 1390
    Illegal XhoI site found at 1343
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1359
    Illegal XbaI site found at 1402
    Illegal PstI site found at 1364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1359
    Illegal XbaI site found at 1402
    Illegal PstI site found at 1364
    Illegal NgoMIV site found at 1972
    Illegal NgoMIV site found at 3255
    Illegal NgoMIV site found at 3538
    Illegal AgeI site found at 600
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References